想要獲得高質(zhì)量蛋白晶體一般需要克服3個(gè)難題：1、獲得純度高的，均一的蛋白樣品；2，篩選可結(jié)晶的條件；3，優(yōu)化結(jié)晶條件，最后獲得高質(zhì)量的單晶；其中，篩選可結(jié)晶的條件是獲得蛋白質(zhì)晶體的主要瓶頸之一；

    Jena Biosource品牌中有一系列蛋白結(jié)晶研究所要用的試劑和耗材，以下給大家介紹一下Jena里面暢銷(xiāo)的蛋白結(jié)晶條件篩選試劑盒。

1、JBScreen Basic

JBScreen Basic是基于稀疏矩陣法的蛋白結(jié)晶試劑盒，包含了96種篩選條件，整套試劑盒分為4個(gè)小規(guī)格，每個(gè)小規(guī)格包含24種條件，24個(gè)條件分別密封在螺旋蓋試管中，每管10ml,可以隨時(shí)使用。Jena里面的蛋白結(jié)晶篩選試劑盒不包含二甲砷酸鹽，用MES進(jìn)行替代。

二甲砷酸鹽，含砷，是有毒物質(zhì)

1、      致癌物質(zhì)；

2、      吸入后對(duì)人體有害，刺激眼睛和皮膚，長(zhǎng)時(shí)間接觸，可導(dǎo)致腎臟和肝臟受損；

3、      能與自由巰基反應(yīng)，可以與半胱氨酸硫共價(jià)結(jié)合，所以存在蛋白構(gòu)象受到嚴(yán)重影響的風(fēng)險(xiǎn)；

2、 JBScreen Classic

      這個(gè)系列是Jena品牌中最初推出的經(jīng)典的結(jié)晶條件試劑盒，在文獻(xiàn)中的應(yīng)用以每年30%的速度增長(zhǎng)；這個(gè)試劑盒包含了240個(gè)篩選條件，涵蓋了各種有效沉淀劑和Buffer。整套試劑盒分成了10小規(guī)格，每個(gè)規(guī)格包含了24個(gè)條件，如果只想要其中某些條件，是可以單獨(dú)購(gòu)買(mǎi)小規(guī)格試劑盒的；

         3、JBScreen pentaerythritol

      基于兩種新奇的沉淀劑，季戊四醇丙氧基化物和季戊四醇乙基化物，用于生物大分子最初結(jié)晶條件的篩選。兩者都包含一個(gè)季戊四醇的支鏈型高分子。因此他們不同于傳統(tǒng)的沉淀劑（如MPD和PEG）。另外，季戊四醇聚合物具有冷凍保護(hù)劑的功能，蛋白晶體在這些高濃度的沉淀劑中生長(zhǎng)，并能夠由晶滴直接冷凍；

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